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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Peripheral B-Cell Subset Distribution in Primary Antiphospholipid Syndrome
doi: 10.3390/ijms19020589
Figure Lengend Snippet: B-cell differentiation subsets in patients with primary antiphospholipid syndrome (pAPS), patients with systemic lupus erythematosus (SLE), and healthy controls (HC). Percentages of different B-cell subsets: transitional-immature (CD19 + CD5 + CD10 + CD27 − IgD ++ CD24 hi CD38 hi ), naïve (CD19 + CD5 +/− CD10 − CD27 − IgD + CD24 int CD38 low / − ), non-switched memory (CD19 + CD27 + IgD + CD38 − IgM + ), double-negative (CD19 + CD27 − IgD − CD24 − CD38 −/+ IgM − ), switched memory (CD19 + CD27 + IgD − CD38 + IgM +/− ), and plasma cells(CD19 lo CD27 hi IgD − CD38 hi CD138 −/+ ) in HC and in patients with pAPS or SLE. CD19 + cells were gated for analysis. Box plots show the median and interquartile range. Differences were significant when p value <0.05 by Mann–Whitney U test. HC: healthy controls; pAPS: primary antiphospholipid syndrome; SLE: systemic lupus erythematosus.
Article Snippet: Briefly, PBMCs were stained on surface with the following specific fluorochrome-conjugated monoclonal antibodies: anti-CD27 FITC (clone M-T-271), anti-CD24 FITC and PE (clone ML5), anti-IgD PE (clone A6-2), anti-CD5 PerCP Cy5.5 (clone L17F12),
Techniques: Cell Differentiation, MANN-WHITNEY
Journal: iScience
Article Title: CD38 restrains the activity of extracellular cGAMP in a model of multiple myeloma
doi: 10.1016/j.isci.2024.109814
Figure Lengend Snippet:
Article Snippet: The following antibodies were used for
Techniques: Virus, Isolation, Recombinant, Enzyme-linked Immunosorbent Assay, Protein Extraction, Labeling, Sequencing, Plasmid Preparation, Software, Staining
Journal: PLoS ONE
Article Title: Diffuse Large B Cell Lymphoma Cell Line U-2946: Model for MCL1 Inhibitor Testing
doi: 10.1371/journal.pone.0167599
Figure Lengend Snippet: Immunoprofile of patient and–derived cell line U-2946.
Article Snippet: Antibodies (Abs) against CD3 (Leu4), CD5 (UCHT2), CD10 (HI10a), CD19 (Leu12), CD20 (Leu16), CD34 (clone 581), CD37 (M-B371), CD38 (HIT2),
Techniques:
Journal: Frontiers in Immunology
Article Title: The impact on colostrum oxidative stress, cytokines, and immune cells composition after SARS-CoV-2 infection during pregnancy
doi: 10.3389/fimmu.2022.1031248
Figure Lengend Snippet: List of antibodies used for immunophenotyping.
Article Snippet:
Techniques:
Journal: Frontiers in Immunology
Article Title: The impact on colostrum oxidative stress, cytokines, and immune cells composition after SARS-CoV-2 infection during pregnancy
doi: 10.3389/fimmu.2022.1031248
Figure Lengend Snippet: Cell populations in the colostrum of symptomatic (n=14) and asymptomatic (n=12) for Covid-19 of lactating women, during pregnancy. (A) IgA colostrum concentration (ng mL -1 ). (B) Total cell count (x10 7 mL -1 ). (C) Percentage of cells CD3-CD19 - CD138 + CD27 ++ . (D) Percentage of cells CD3 - CD19 + CD138 - CD27 - . (E) Percentage of cells CD3 - CD19 + CD138 - CD27 + . (F) Percentage of cells CD3 + CD4 + CD45RO - CD62L + . (G) Percentage of cells CD3 + CD8 + CD45RO - CD62L + . (H) Percentage of cells CD3 + CD4 + CD45RO + CD62L + . (I) Percentage of cells D3 + CD8 + CD45RO + CD62L + . (J) Percentage of cells CD3 + CD4 + CD45RO + CD62L - . (L) Percentage of cells CD3 + CD8 + CD45RO + CD62L - . (K) Percentage of cells CD3 + CD4 + CD45RO - CD62L - . (M) Percentage of cells CD3 + CD8 + CD45RO - CD62L - . (N) Percentage of cells CD3 + CD4 + Tbet + . (O) Percentage of cells CD3 + CD4 + GATA3 + . (P) Percentage of cells CD3 + CD4 + RORγt + IL17A + . (Q) Percentage of cells CD3 - CD4 - Tbet + . (R) Percentage of cells CD3 - CD4 - RORγt + . (S) Percentage of cells CD3 - CD4 - GATA3 + . (T) Percentage of cells CD3 - CD56 bright CD16 - CD27 + IFN-γ + . (U) Percentage of cells CD3 - CD56 dim CD16 + CD27 - . Data are presented as median and 95% CI. Mann–Whitney test: p < 0.05. * p < 0.000. ** p = 0.0093.
Article Snippet:
Techniques: Concentration Assay, Cell Counting, MANN-WHITNEY
Journal: Frontiers in Immunology
Article Title: The impact on colostrum oxidative stress, cytokines, and immune cells composition after SARS-CoV-2 infection during pregnancy
doi: 10.3389/fimmu.2022.1031248
Figure Lengend Snippet: Gate strategies and representative dot plot graphs from flow cytometry data of two women (Asymptomatic number 7 and Symptomatic number 4). (A) CD3 - CD19 - and CD3 - CD19 + cells were gated for CD138 and CD27 analyzis. (B) CD3 + cells were further evaluated for CD4 or CD8. CD3 + CD4 + and CD3 + CD8 + cells were gated for CD45RO and CD62L analyzis. (C) CD3 - cells were evaluated for CD56. Both CD56 bright and CD56 dim were further analyzed for CD16 and CD27 expression. CD3 - CD56 bright CD16 - CD27 + cells were evaluated for IFN-γ analyzis. (D) CD3 + CD4 + and CD3 - CD4 - were evaluated for tbet, GATA3, and RORγt. CD3 + CD4 + RORγt + were also analyzed for IL17A.
Article Snippet:
Techniques: Flow Cytometry, Expressing